An eight-plex immunoassay for Group A streptococcus serology and vaccine development

AL Whitcombe, F Han, SM McAlister… - Journal of …, 2022 - Elsevier
develop a multiplex beadbased immunoassay that can detect and quantify antibody responses
Quantification of IgG antibodies and standardisation between assay runs was achieved …

Development and Evaluation of a New Triplex Immunoassay That Detects Group A Streptococcus Antibodies for the Diagnosis of Rheumatic Fever

AL Whitcombe, P Hanson-Manful, S Jack… - Journal of Clinical …, 2020 - Am Soc Microbiol
… in antibody response. Accordingly, our objective was to … design and optimize the multiplex
immunoassay technology for the … Forty-five of these participants were excluded from analysis, …

Novel Multiplex Immunoassays for Quantification of IgG against Group B Streptococcus Capsular Polysaccharides in Human Sera

G Buffi, B Galletti, M Stella, D Proietti, E Balducci… - Msphere, 2019 - Am Soc Microbiol
… for the simultaneous determination of antibody responses to … Seventeen of the analyzed
sera showed mean fluorescence … to develop two microsphere multiplex immunoassays based …

Multilaboratory comparison of pneumococcal multiplex immunoassays used in immunosurveillance of Streptococcus pneumoniae across Europe

B Meek, N Ekström, B Kantsø, R Almond, J Findlow… - Msphere, 2019 - Am Soc Microbiol
… of vaccine-induced antibody responses. However, some serotype-… techniques, have been
developed in various laboratories … determination of vaccine-induced pneumococcal antibodies

Multiplexed suspension array immunoassays for detection of antibodies to pneumococcal polysaccharide and conjugate vaccines

S Dunbar - Frontiers in Cellular and Infection Microbiology, 2023 - frontiersin.org
… This article reviews publications describing the development … the fluorescence of the internal
dyes allows distinct analysisgroups showed greater antibody responses than the control …

Novel multiplex immunoassays for two important infectious diseases in Aotearoa New Zealand

AL Whitcombe - 2022 - researchspace.auckland.ac.nz
… -based multiplex immunoassays to study antibody responses … Multi-antigen analysis revealed
striking differences in the … thesis, where I developed a multiplex serological assay for SARS…

[HTML][HTML] Antibody enhanced HPLC for serotype-specific quantitation of polysaccharides in pneumococcal conjugate vaccine

JZ Deng, N Kuster, A Drumheller, M Lin, F Ansbro… - npj Vaccines, 2023 - nature.com
… A fluorescence-labeled multiplex assay format has also been … and over 30 of them can
cause streptococcus infections 1,2 . … our PCV development, we sought to develop an innovative …

Increased Breadth of Group A Streptococcus Antibody Responses in Children With Acute Rheumatic Fever Compared to Precursor Pharyngitis and Skin Infections

AL Whitcombe, R McGregor, J Bennett… - The Journal of …, 2022 - academic.oup.com
… Robust, high-throughput assays based on advanced multiplexdeveloped a triplex
immunoassay to measure antibody … syndromes, a focused analysis was performed on participants …

Kinetics of the severe acute respiratory syndrome coronavirus 2 antibody response and serological estimation of time since infection

S Pelleau, T Woudenberg, J Rosado… - The Journal of …, 2021 - academic.oup.com
… We developed a multiplex serological test for measuring … In this study, we use multiplex
assays to measure antibodies to … the median fluorescence intensity (MFI) was used for analysis. …

[HTML][HTML] Development of A Standardized Opsonophagocytosis Killing Assay for Group B Streptococcus and Assessment in an Interlaboratory Study

S Leung, CF Collett, L Allen, S Lim, P Maniatis… - Vaccines, 2023 - mdpi.com
… in the development of a multiplexed (Luminex) immunoassayquantification of OPKA activity
in samples with low antibody … pHrodo, a fluorescent dye that increases in fluorescence in the …